Biomolecular NMR Facility - Tutorials

FOR VANDERBILT USE ONLY!

  • Nitrogen Fill Tutorial (M. Voehler 2001)
  • Nitrogen Fill Tutorial - pdf version (M. Voehler 2001)
  • nmrPipe from CSB

    VANDERBILT NMR Tutorials!

    (All tutorials are witten for Topspin)
    Format: pdf files with bookmarks and linked index

    General Acquisition Tutorials

  • 90 deg 1H Pulse Calibration Tutorial (02/2008)
  • Tuning Tutorial (12/2008)
  • How to use the Autosampler (02/2008)
  • How to change the mode on the SampleJet (07/2012)

    General Processing Tutorials

  • 2D Signal to Noise Calculation (01/2013)
  • 13C Baseline Correction on High Q Probes (01/2013)

    1D Tutorials

    General Topspin 1D Manual (CSB)
  • TS 3.x 1D guide for all spectrometers (6/2018)
  • How to use the PlotEditor (Bruker Manual 2015)

    • Instrument Specific Topspin 1D Manual (SMF)
  • DPX300, 1H, 13C, 19F and 31P (02/2008)
  • DRX400, 1H, 13C and 11B (03/2011)
  • DRX500, 1H, 13C and 31P (03/2011)

    2D Tutorials

    Homonuclear 2D Experiments
    A guide for all VU spectrometers (06/2008)

    Parameters are given for the following experiments with and without water suppression.
  • COSY: magnitude or phase sensitive
  • TOCSY: phase sensitive
  • NOESY: phase sensitive

    Heteronuclear 2D Experiments
    A guide for all VU spectrometers (06/2008)

    Parameters are given for the following 13C / 1H correlated experiments.

  • HSQC: Multiplicity edited, phase sensitive
  • HMBC: Magnitude
  • H2BC: selective for 2 bond C-H correlations, phase sensitive