502: 5mm CP-TXI-Z, #Z44861-0086 TXI500S2-HC/N-D 5Z

In principle: Do NOT trust these values blindly - verify them if questionable!

Amplifiers can go bad or your sample environment like salt, temperature, ... might alter the pulse width!

IMPORTANT POWER REFERENCES FOR CRYOPROBES (full document)

4.2 Pulse and power recommendations

 

Note: All values require a minimum repetition period d1 +aq of 1sec 2)

Important: observe the separate notes on the foIIowing pages!

Important: Be CAREFUL when pushing these limits!

 

 

5mm 500 MHz TXI CryoProbe TM

1H

 

Hard pulse 3)

(max. length 360o)

8 usec

Hard pulse for lossy sample

Power level corresponding to 8.0 usec pulse for non-lossy samples

trim pulse p28 4)

2 msec @ 10 usec

TOCSY spin lock

200 msec @ 25 usec

400 msec @ 35 usec

ROESY spin lock

Up to CW for power level

corresponding to a 100 usec pulse

WALTZ 16 decoupling

during 13C-detection

Up to CW for power level corresponding to a 100 usec pulse

DIPSI2-decoupling in triple resonance

400 msec @ 50 usec

13C

 

hard pulse 6)

(max. length 360o)

15.0 usec

trim pulse 5)

2msec @ 25 usec

CC spin lock 5)

20msec @ 25 usec

GARP decoupling 7)

140msec @ 65 usec

selective pulses 8)

G4: 320 usec

Q3: 256 usec

CHIRP: 2ms @ 25 usec

15N

 

hard pulse 6)

(max. length 360o)

40.0 usec

GARP4 decoupling 7)

140 msec @ 200 usec

CPMG T2 9)

250 msec @ 80 usec (see warning 9))

2H

 

hard pulse

(max. length 360o)

150 usec

WALTZ16 decoupling

100 msec @ 250 usec

Z-Gradient

 

Absolute max. current 10)

10 A

Max. overall length 11)

10 ms @ 10A

 

Date: 25.10.2004

1. General Information:

  • Shimfile: chloshim.600 is a good starting point for aqueous samples
  • Read Pulses from Master List:
    • Read in pulse sequence and Parameter file
    • Use the getprosol command or click on the test tube symbol in ased
    • For Bruker Pulse Sequences type: getprosol 1H 'p1' ' pl1' , where p1 is the determined pulse length [us] at the power level pl1 [dB].

2. Hard Pulses:

Nucleus Type Attenuation [dB] Channel 90deg Pulse Power [W] Calc. Date
1H -9.0 dB f1 8 us 9.0 W 1/12/17
waltz16 f1 80.0 us
TOCSY f1 26.0 us
ROESY f1 90.0 us
.
13C hard -18.45 dB f2 15.0 us 70.0 W 1/12/17
AcGly TOCSY -13.85 dB f2 25.0 us 24.3 W -14.01 dB 1/12/17
Dec -5.50 dB f2 65.0 us 3.55 W -5.71 dB 1/12/17
LowDec 1 -3.70 dB f2 80.0 us 2.34 W -3.91 dB 1/12/17
LowDec 2 +2.40 dB f2 160.0 us 0.58 W+2.11 dB 1/12/17
.
15N hard -17.75 dB f3 45.0 us 59.6 W 1/12/17
Urea -11.75 dB f3 90.0 us 15.0 W -11.73dB 1/12/17
-2.95 dB f3 240.0 us 1.97 W -3.21 dB 1/12/17
.
2H -13.15 dB 2HTX 100 us 20.6 W 5/14/16
-4.73 dB 2HTX 250 us 2.97 W -5.19 dB 5/14/16

3. Soft Pulses: (AcGly)

Nucleus Type Channel Pulse Attenuation Amplifier Calc. Date
13C, α+β Q5.1000 384us f2 90 deg -15.05 dB (-15.31)32.0 W on res. 1/12/17
α+β Q5tr.1000 384us f2 90 deg -15.25 dB (-15.31)33.5 W on/off res. 1/12/17
α Q5.1000 1800us f2 90 deg -1.55 dB (1.89)1.43 W on/off res. 1/12/17
α Q5tr.1000 1800us f2 90 deg -1.70 dB (1.89)1.48 W on/off res. 1/12/17
α+β Q3.1000 307us f2 180 deg -14.00 dB (-14.39)25.1 W on res. 1/12/17
α Q3.1000 840us f2 180 deg -5.30 dB (-5.65)3.39 W on res. 1/12/17
CO dec Q3.1000 1200us f2 180 deg -2.10 dB (-2.55)1.62 W on res. 1/12/17
CO Sinc1.1000 160us f2 90 deg offs=0ppm +14.90 dB 07/20/06
CO Sinc1.1000 160us f2 90 deg offs=136ppm +14.90 dB 07/20/06
CO Sinc1.1000 160us f2 180 deg offs=0ppm +9.25 dB 07/20/06
CO Sinc1.1000 160us f2 180 deg offs=136ppm +9.30 dB 07/20/06
adiabatic:α+β hsec.3 500us f2 180 deg +5.5 dB 1/16/04
adiabatic: CO sech1024_4.8 600us f2 180 deg +10.0 dB 1/16/04
1H sinc1.1000 1.2ms f1 180 deg

4. Miscellaneous:

Gradient Strength on Z: 54.584 G/cm 100%, (5mm phantom, rectangular gradient)
Offset Ca - carbonyl (56 - 175ppm): -14965 Hz
1H offset @ 4.7 pppm: 2350.6 Hz
Main Field: 500.13 MHz
BSMS Field: +2966 units (10-13-2003)

5. Conditions for pulse determination:

  • Sample:
    1H-pulses: 2mM Sucrose + 0.5mM DSS + 2mM NaN3, 90% H2O 10% D2O
    X-pulses: 15N + 2H labled Urea in DMSO
    15N-shift: 76.0ppm, J=88.5Hz
    13C: NaAc in D2O
    13C-shift: 21.0ppm, J=128.0Hz
  • Temperature: 298K
  • RF-Filters: none
    1H = none
    X = none
    Y = none
    Z = none
  • Attenuators at amplifier input: none

    POWERCHECK DONE

    1H = 0dB
    X = 0dB
    Y = 0dB
    Z = 0dB
  • Cortab combinations done 1/16/04:
    1H --> FCU 1+2, MOD, MULT, BLA
    13C --> FCU 1, 2, 3, 4, on X-Amplifier: MOD, MULT, BLA, FCU4 on Z amplifier BLA
    15N --> FCU 3 on Y-Amplifier: MOD, MULT, BLA, FCU4 on Z amplifier BLA