900: 5 mm CPTCI 1H-13C/15N/D Z-GRD (Oct 2011) #Z117770-0002

In principle: Do NOT trust these values blindly - verify them if questionable!

Amplifiers can go bad or your sample environment like salt, temperature, ... might alter the pulse width!

IMPORTANT POWER REFERENCES FOR CRYOPROBES (full document)

4.2 Pulse and power recommendations

 

Note: All values require a minimum repetition period d1 +aq of 1sec 2)

Important: observe the separate notes on the foIIowing pages!

Important: Be CAREFUL when pushing these limits!

 

 

5mm 900 MHz TXI/TCI CryoProbe TM

1H

 

Hard pulse 3)

(max. length 360o)

8 µs

Hard pulse for lossy sample

Power level corresponding to 8.0 µs pulse for non-lossy samples

trim pulse p28 4)

2 msec @ 11 µs

TOCSY spin lock

120 msec @ 20 µs

400 msec @ 35 µs

ROESY spin lock

Up to CW for power level

corresponding to a 80 µs pulse

WALTZ 65 decoupling

during 13C-detection

Up to CW for power level corresponding to a 80 µs pulse

DIPSI2-decoupling in triple resonance

400 msec @ 35 µs

13C

 

hard pulse 6)

(max. length 360o)

12.0 µs

trim pulse 5)

2msec @ 22 µs

CC spin lock 5)

20msec @ 22 µs

GARP-4 decoupling 7)

140msec @ 55 µs [110 ppm bandwidth]

Adiabatic Decoupling

140msec @ 55 µs
Crp48,1.5,25.2 [>160 ppm bandwidth]

Band selective pulses 8)

G4: 274 µs

Q3: 172 µs

CHIRP: 2ms @ 22.2 µs

15N

 

hard pulse 6)

32.0 µs

GARP-4 decoupling 7)

140 msec @ 170 µs

CPMG T2 9)

250 msec @ 40 µs (see warning 9))

2H

 

hard pulse

(max. length 360o)

150 µs

WALTZ64 decoupling

100 msec @ 250 µs

Z-Gradient

 

Absolute max. current 10)

10 A

Max. overall length 11)

10 ms @ 10A

 

Valid from the date of shipment: January first 2008. For older CryoProbe please contact your Bruker office.

1. General Information:

  • Shimfile: chloshim.600 is a good starting point for aqueous samples
  • Read Pulses from Master List:
    • Read in pulse sequence and Parameter file
    • Use the getprosol command or click on the test tube symbol in ased
    • For Bruker Pulse Sequences type: getprosol 1H 'p1' ' pldb1' , where p1 is the determined pulse length [us] at the power level PldB1 [dB].

    2. Hard Pulses:

    (80 W max)
    Nucleus Type 90deg Pulse Channel Attenuation [dB] Power[W] Calc. Date
    1H 8.00 us f1 -11.5 dB 14.1 W 06/10/21
    waltz16 55.0 us f1 getprosol
    TOCSY 24.0 us f1 getprosol
    ROESY 125.0 us f1 getprosol
    .
    13C hard 12 us f2 -22.60 dB 182.0 W 06/10/21
    AcGly TOCSY 25 us f2 -16.20 dB 41.69 W -16.22 dB 06/10/21
    cpd 50 us f2 -10.25 dB 10.59 W -10.20 dB 06/10/21
    LowDec 1 70.5 us f2 -7.20 dB 5.25 W -7.22 dB 06/10/21
    .
    15N hard 32 us f3 -24.80 dB 302.0 W 06/10/21
    80 us f3 -17.30 dB 53.7 W -16.84 dB 06/10/21
    100 us f3 -15.40 dB 34.7 W -14.90 dB 06/10/21
    220 us f3 -8.30 dB 6.76 W -8.05 dB 06/10/21
    310 us f3 -5.40 dB 3.47 W -5.08 dB 06/10/21
    350 us f3 -4.30 dB 2.69 W -4.02 dB 06/10/21
    .
    2H 80 us f1 -18.10 dB 64.56 W 10/28/20
    C6D6, Cracac 80 us f4 -17.80 dB 60.2 W 10/28/20
    190 us f4 -10.00 dB 10.0 W-10.29 dB 10/28/20
    250 us f4 -7.70 dB 5.89 W -7.90 dB 10/28/20

    3. Soft Pulses:

    Nucleus Type Channel Pulse Attenuation [dB] Power [W] Resonance Date
    13C , Cali/CO G4.256 273us f2 90 deg -20.50 dB
    (-20.88)    		112.2 W on 06/10/21
    Cali/CO G4tr.256 273us f2 90 deg -20.50 dB
    (-20.88)    		 112.2 W on 06/10/21
    Cα Q5.1000 1000us f2 90 deg -9.20 dB
    (-9.45)    		 8.32 W on 06/10/21
    Cα Q5tr.1000 1000us f2 90 deg -9.35 dB
    (-9.45)    		8.61 W on 06/10/21
    Cali/CO Q3.1000 171us f2 180 deg -21.80 dB
    (-21.94)    		 151.4 W on 06/10/21
    Cα Q3_surbop.1 700us f2 180 deg -11.35 dB
    (-11.68)    		 13.7 W on 06/10/21
    med sel Q3_surbop.1 493us f2 180 deg -14.45 dB
    (-14.73)    		27.9 W on 06/10/21
    high sel Q3_surbop.1 1400us f2 180 deg -5.50 dB
    (-5.66)    		3.55 W on 06/10/21
    13C direct hard 12 us f1 90 deg -21.85 dB 156.7 W 07/3/20
    Q5.1000 213us f1 90 deg -22.05 dB (-22.14) 160.3 W on 07/3/20
    CO Q5tr.1000 213us f1 90 deg -22.10 dB (-22.14) 162.2 W on 07/3/20
    CO Q3.1000 171us f1 180 deg -21.40dB (-21.19) 138.4 W on 07/3/20

    4. Miscellaneous:

    Offset Ca - carbonyl (56 - 175ppm): -26936.3 Hz
    1H offset @ 4.7 pppm: 4230.94 Hz
    Main Field: 900.204 MHz
    BSMS Field: units (10-10-2011)

    5. Conditions for pulse determination:

    • Sample: 13C, 15N labled Urea in DMSO
      13C-shift: 48.5ppm, J=138.5Hz
      15N-shift: 76.0ppm, J=88.5Hz
      Acetyl Glycine 13C/15N, in D2O
      X-pulses: 13C, 15N labled Acetyl Glycine
      13C-shift: 22.1ppm, J=128.6Hz (1H 1.96 ppm )
      15N-shift: 76.0ppm, J=88.5Hz