800: 5mm TXI-Z, #550501-0011

In principle: Do NOT trust these values blindly - verify them if questionable!

Amplifiers can go bad or your sample environment like salt, temperature, ... might alter the pulse width!

1. General Information:

  • Shimfile: 550501_011ws.shim is a good starting point for aqueous samples

2. Hard Pulses:

Nucleus Type Attenuation Channel 90deg Pulse Amplifier Calculated Date
1H -1.0 dB f1 9.4 us 100W 11/23/05
waltz16 f1 70.0 us 100W
TOCSY f1 25-35.0 us 100W
ROESY f1 100.0 us 100W
.
13C hard -3.3 dB f2 15.0 us 500W 11/23/05
Spec -5.2 dB f2 11.0 us 500W dB 2/17/05
TOCSY dB f2 us 500W dB
Dec dB f2 54.0 us 500W dB
LowDec 1 +11.0 dB f2 80.0 us 500W dB 2/17/05
LowDec 2 dB f2 160.0 us 500W dB
.
15N hard -5.9 dB f3 40.0 us 300W 2/17/05
-5.2 dB f3 45.0 us 300W dB 11/23/05
+4.0 dB f3 150.0 us 300W dB 2/17/05
+7.0 dB f3 200.0 us 300W dB 11/23/05
.
2H -1.5 dB f4 (lockswitch) 90.0 us 300W 2/17/05
+8.75 dB f4 (lockswitch) 250 us 300W 2/17/05

3. Soft Pulses:

Nucleus Type Channel Pulse Attenuation Amplifier Calculated Date
13C G4.256 340us f2 90 deg dB500W
G4.256 680us f2 90 deg dB500W
G3.256 200us f2 180 deg dB500W
G3.256 640us f2 180 deg dB500W
G4tr.256 340us f2 90 deg dB500W
Sinc1 160us f2 90 deg dB500W
Sinc1 160us f2 180 deg dB500W
1H Sinc1.1000 1.2ms f1 180 deg dB100W

G3 and G4 pulses were determined with spoal=0.5, on-resonance

4. Miscellaneous:

Magic angle on GREAT III: factor Z/XY = N/A
Offset Ca - carbonyl (56 - 175ppm): -18109Hz
1H offset @ 4.7 pppm: 2820 Hz
Main Field: 800.19 MHz

5. Conditions for pulse determination:

  • Samples:
    1H-pulses: 2mM Sucrose + 0.5mM DSS + 2mM NaN3, 90% H2O 10% D2O
    X-pulses: 13C, 15N labled Urea in DMSO
    13C-shift: 48.5ppm, J=138.5Hz
    15N-shift: 76.0ppm, J=88.5Hz
  • Temperature: 298K
  • RF-Filters:
    1H = none
    X = Highpass 800MHz 13C-Pass; 2H-Stop
    Y = Lowpass 800MHz 15N-pass; 2H-Stop
    Z = none
  • Attenuators at amplifier input: none
    1H = 4dB
    X = 4dB
    Y = 0dB
    Z = 0dB
  • Cortab combinations done 3-27-2002 (mc) XWIN-NMR 3.0:
    1H: --> FCU 1+2, Mult, Mod, BLA, redone after amplifier change 1-13-2004
    13C:
    FCU 1+2, 500_1: Mult, Mod, BLA
    FCU 3+4, 300_5: Mult, Mod, BLA, 300_6, 500_1: BLA
    15N:
    FCU 1+2, 500_1: Mult, Mod, BLA
    FCU 3, 300_5: Mult, Mod, BLA, 300_6, 500_1: BLA
    Modify the file: /u/exp/stan/nmr/lists/cpd.hwt/cortab :
    At the end of the file add the line "jump to 1", add 4ms delay.
    Do this only for Amplifier Cortab!