800: 5mm CP-TCI-Z, #44909-0018,
CP-TCI 800SG H-C/N-D-05-Z

In principle: Do NOT trust these values blindly - verify them if questionable!

Amplifiers can go bad or your sample environment like salt, temperature, ... might alter the pulse width!

IMPORTANT POWER REFERENCES FOR CRYOPROBES (full document)

4.2 Pulse and power recommendations

 

Note: All values require a minimum repetition period d1 +aq of 1sec 2)

Important: observe the separate notes on the foIIowing pages!

Important: Be CAREFUL when pushing these limits!

 

 

5mm 800 MHz TXI CryoProbe TM

1H

 

Hard pulse 3)

(max. length 360o)

8 usec

Hard pulse for lossy sample

Power level corresponding to 8.0 usec pulse for non-lossy samples

trim pulse p28 4)

2 msec @ 10 usec

TOCSY spin lock

 

ROESY spin lock

200 msec @ 25 usec

400 msec @ 35 usec

Up to CW for power level

corresponding to a 80 usec pulse

WALTZ 16 decoupling

during 13C-detection

Up to CW for power level corresponding to a 100 usec pulse

DIPSI2-decoupling in triple resonance

400 msec @ 35 usec

13C

 

hard pulse 6)

(max. length 360o)

15.0 usec

trim pulse 5)

2msec @ 22 usec

CC spin lock 5)

20msec @ 22 usec

GARP decoupling 7)

140msec @ 55 usec

selective pulses 8)

G4: 308 usec

Q3: 210 usec

CHIRP: 2ms @ 25 usec

15N

 

hard pulse 6)

(max. length 360o)

40.0 usec

GARP4 decoupling 7)

140 msec @ 170 usec

CPMG T2 9)

250 msec @ 80 usec (see warning 9))

2H

 

hard pulse

(max. length 360o)

150 usec

 

 

WALTZ16 decoupling

100 msec @ 250 usec

Z-Gradient

 

Absolute max. current 10)

10 A

Max. overall length 11)

10 ms @ 10A

 

Date: 25.10.2004

1. General Information:

  • Shimfile: chloshim.600 is a good starting point for aqueous samples
  • Read Pulses from Master List:
    • Read in pulse sequence and Parameter file
    • Use the getprosol command or click on the test tube symbol in ased
    • For Bruker Pulse Sequences type: getprosol 1H 'p1' ' pl1' , where p1 is the determined pulse length [us] at the power level pl1 [dB].

2. Hard Pulses: AVIII

Nucleus Type Attenuation[dB] Channel 90 deg Pulse Power[W] Expected change Date
1H -10.0 dB f1 8.0 us 10.0W 4/14/14
Urea waltz16 f1 65.0 us
TOCSY f1 25.0 us
ROESY f1 80.0 us
.
13C hard -23.15 dB f2 12.0 us 206.5 W 4/14/14
AcGly CC-TOCSY -17.90 dB f2 22.0 us 61.7 W 4/14/14
CC-TOCSY -16.65 dB f2 25.0 us 48.4 W 4/14/14
Dec -11.20 dB f2 48.0 us 13.2 W 4/14/14
LowDec -8.65 dB f2 65.0 us 7.33 W 4/14/14
.
15N hard -24.40 dB f3 37.0 us 275.4 W 4/14/14
-16.90 dB f3 90.0 us 49.9 W 4/14/14
-9.80 dB f3 200.0 us 9.55 W 4/14/14
-5.10 dB f3 350.0 us 3.25 W 4/14/14
.
2H -12.0 dB f4 157 us 15.85 W 2/15/11
-8.4 dB f4 240 us 6.92 W 2/15/11
-6.4 dB f4 300 us 4.34 W 2/15/11

3. Soft Pulses:

Nucleus Type Channel Pulse Attenuation [dB] Power [W] Calc. Date
13C, α+β Q5.1000 260us f2 90 deg -22.0 dB (-21.70)158.5 W on res. 4/14/14
α+β Q5tr.1000 260us f2 90 deg -22.0 dB (-21.70)158.5 W on res. 4/14/14
α Q5.1000 1125us f2 90 deg -10.0 dB (-8.98)10.0 W on/off res. 4/14/14
α Q5tr.1000 1125us f2 90 deg -10.0 dB (-8.98)10.0 W on/off res. 4/14/14
α+β Q3.1000 192us f2 180 deg -21.10 dB (-21.48)128.8 W on res. 4/14/14
α Q3.1000 525us f2 180 deg -12.50 dB (-12.74)17.78 W on res. 4/14/14
CO dec Q3.1000 750us f2 180 deg -9.20 dB (-9.65)8.32 W on res. 4/14/14
CO dec Q3.1000 1500us f2 180 deg -3.00 dB (-3.63)2.00 W on res. 4/14/14
α+β Q5tr.1000 260us f1 90 deg -22.50 dB (-21.90)177.8 W on res. 4/14/14
α+β Q5tr.1000 260us f1 90 deg -22.70 dB (-21.90)186.2 W on res. 4/14/14
α+β Q3.1000 192us f1 180 deg -21.30 dB (-20.98)134.9 W on res. 4/14/14
α+β Q3.1000 750us f1 180 deg -9.65 dB (-9.15)9.2 W on res. 4/14/14
CO dec Q3.1000 750us f2 180 deg -9.30 dB (-9.35)8.51 W on res. 4/14/14
CO Sinc1.1000 160us f2 90 deg +12.50 dB500W dB 11/27/2006
CO Sinc1.1000 160us f2 180 deg +6.5 dB500W dB 11/27/2006
adiabatic:α+β HypSec 500us f2 180 deg -2.0 dB500W N/A 3/18/2005
adiabatic: CO sech1024_4.8 600us f2 180 deg dB500W N/A 1/16/04
1H sinc1.1000 1.2ms f1 180 deg + dB100W

4. Miscellaneous:

Offset Ca - carbonyl (56 - 174ppm):
1H offset @ 4.7 pppm:
Main Field: 800.21 MHz

5. Conditions for pulse determination:

  • Samples:
    1H-pulses: AcetylGlycine in D2O
    X-pulses: 13C, 15N labled NAcG in D2O or H2O
    13C-shift: 22.1ppm, J=129.0Hz
    15N-shift: 115.0ppm, J=95.5Hz
    15N-shift: 76.0ppm, J=90.0Hz (urea)
  • Temperature: 298K
  • RF-Filters:
    1H = none
    X = none
    Y = none
    Z = none
  • Attenuators at amplifier input: none
    1H = 4dB
    X = 4dB
    Y = 0dB
    Z = 0dB
  • Cortab combinations done 3-16-2002 (mc) XWIN-NMR 3.5:
    1H: --> FCU 1+2, Mult, Mod, BLA
    13C:
    FCU 1+2, 500_1: Mult, Mod, BLA
    FCU 3+4, 300_5: Mult, Mod, BLA, 300_6, 500_1: BLA
    15N:
    FCU 3,4 300_5: Mult, Mod, BLA, 300_6, 500_1: BLA
    2H:
    FCU 4 300_5: Mult, Mod, BLA