800: 5mm CP-TCI-Z, #Z122992/0001 5 mm CPTCI 1H-13C/15N/D Z-GRD

In principle: Do NOT trust these values blindly - verify them if questionable!

Amplifiers can go bad or your sample environment like salt, temperature, ... might alter the pulse width!

IMPORTANT POWER REFERENCES FOR CRYOPROBES (full document)

4.2 Pulse and power recommendations

 

Note: All values require a minimum repetition period d1 +aq of 1sec 2)

Important: observe the separate notes on the foIIowing pages!

Important: Be CAREFUL when pushing these limits!

 

 

5mm 800 MHz TCI CryoProbe TM

1H

 

Hard pulse 3)

(max. length 360o)

8 usec

Hard pulse for lossy sample

Power level corresponding to 8.0 usec pulse for non-lossy samples

trim pulse p28 4)

2 msec @ 10 usec

TOCSY spin lock

200 msec @ 25 usec

400 msec @ 35 usec

ROESY spin lock

Up to CW for power level

corresponding to a 80 usec pulse

WALTZ 16 decoupling

during 13C-detection

Up to CW for power level corresponding to a 80 usec pulse

DIPSI2-decoupling in triple resonance

400 msec @ 35 usec

13C

 

hard pulse 6)

(max. length 360o)

12.0 usec

trim pulse 5)

2msec @ 22 usec

CC spin lock 5)

20msec @ 22 usec

GARP decoupling 7)

140msec @ 55 usec

selective pulses 8)

G4: 308 usec

Q3: 192 usec

CHIRP: 2ms @ 22.2 usec

15N

 

hard pulse 6)

(max. length 360o)

35.0 usec

GARP4 decoupling 7)

140 msec @ 170 usec

CPMG T2 9)

250 msec @ 45 usec (see warning 9))

2H

 

hard pulse

(max. length 360o)

150 usec

WALTZ16 decoupling

100 msec @ 250 usec

Z-Gradient

 

Absolute max. current 10)

10 A

Max. overall length 11)

10 ms @ 10A

 

Valid from the date of shipment: January first 2007

1. General Information:

  • Shimfile: chloshim.600 is a good starting point for aqueous samples
  • Read Pulses from Master List:
    • Read in pulse sequence and Parameter file
    • Use the getprosol command or click on the test tube symbol in ased
    • For Bruker Pulse Sequences type: getprosol 1H 'p1' ' pldb1' , where p1 is the determined pulse length [us] at the power level pldb1 [dB].

2. Hard Pulses:

Nucleus Type 90deg Pulse Channel Attenuation Power [W] Calc. Date
1H 8.0 us f1 -11.00 dB 12.59 W 11/10/25
waltz16 60.0 us f1
TOCSY 24.0 us f1
ROESY 80.0 us f1
.
13C hard 12.0 us f2 -21.30 dB 134.9 W 11/10/25
TOCSY 25.0 us f2 -15.20 dB 33.1 W (-14.92) 11/10/25
Dec 50.0 us f2 -9.15 dB 8.22 W (-8.90) 11/10/25
LowDec 70.5 us f2 -6.15 dB 4.16 W (-5.92) 11/10/25
LowDec 2 100.0 us f2 -3.05 dB 2.01 W (-2.88) 11/10/25
.
15N hard 32 us f3 -23.80 dB 239.9 W 11/10/25
Urea Dec 220 us f3 -7.20 dB 5.25 W (-7.05) 11/10/25
Dec 310 us f3 -4.05 dB 2.54 W (-4.08) 11/10/25
Dec 350 us f3 -3.00 dB 2.00 W (-3.02) 11/10/25
Dec 80 us f3 -16.10 dB 40.7 W (-15.84) 11/10/25
.
2H 70 us f1 -21.50 dB 141.3 W 11/10/20
C6D6 70 us f4 -21.50 dB 141.3 W 11/10/20
250.0 us f4 -10.55 dB 11.4 W (10.44) 11/10/20

3. Soft Pulses:

Nucleus Type Channel Pulse Attenuation (Calculated) Power [W] Resonance Date
13C , Cali/CO; Q5.1000 240us f2 90 deg -20.70 dB (-20.55)117.5 W on 11/10/25
Cali/CO Q5tr.1000 240us f2 90 deg -20.90 dB (-20.55) 123.0 W on 11/10/25
Cα Q5.1000 1125us f2 90 deg -7.20 dB (-7.13) 5.25 W on 11/10/25
Cα Q5tr.1000 1125us f2 90 deg -7.35 dB (-7.13)5.43 W on 11/10/25
Cali/CO Q3.1000 192us f2 180 deg -19.70 dB (-19.63) 93.3 W on res 11/10/25
Cali/CO Q3_surbop.1 788us f2 180 deg -9.15 dB (-9.36) 8.22 W
11/10/25
CO dec Q3_surbop.1 555us f2 180 deg -12.20 dB (-12.40) 16.6 W on 11/10/25
CO dec Q3_surbop.1 1575us f2 180 deg -3.20 dB (-3.34)2.09 W on 11/10/25
13C direct detect
13C hard, 12us f1 -20.90 dB 123.0 W 05/11/15
Cali/CO Q5.1000 240us f1 90 deg -20.70 dB (-20.15)117.5 W on 05/11/15
Cali/CO Q5tr.1000 240us f1 90 deg -20.80 dB (-20.15)120.2 W on 05/11/15
Cα Q5.1000 1125us f1 90 deg -7.10 dB (-6.73)5.13 W on 05/11/15
Cα Q5tr.1000 1125us f1 90 deg -7.30 dB (-6.73)5.37 W on 05/11/15
Cali/CO Q3.1000 192us f1 180 deg -19.50 dB (-19.23)89.1 W on 05/11/15
Cali/CO Q3.1000 768us f1 180 deg -7.60 dB (-7.19)5.75 W
11/10/25
CO dec Q3.1000 525us f1 180 deg -10.80 dB (-10.49)12.0 W on 05/11/15

5. Conditions for pulse determination:

  • Sample:
    1H-pulses: 2mM Sucrose + 0.5mM DSS + 2mM NaN3, 90% H2O 10% D2O
    X-pulses: 15N + 2H labled Urea in DMSO
    15N-shift: 76.0ppm, J=88.5Hz
    13C: NaAc in D2O
    13C-shift: 21.0ppm, J=128.0Hz
  • Temperature: 298K
  • RF-Filters: none
    1H = none
    X = none
    Y = none
    Z = none
  • Attenuators at amplifier input: none

    POWERCHECK DONE

    1H = 0dB
    X = 0dB
    Y = 0dB
    Z = 0dB
  • Cortab combinations done 1/16/04:
    1H --> FCU 1+2, MOD, MULT, BLA
    13C --> FCU 1, 2, 3, 4, on X-Amplifier: MOD, MULT, BLA, FCU4 on Z amplifier BLA
    15N --> FCU 3 on Y-Amplifier: MOD, MULT, BLA, FCU4 on Z amplifier BLA