602: 5mm CPQCI 1H-31P/13C/15N/D Z-GRD (Aug 2014) #Z132808/0002

1. General Information
2. Hard Pulses
3. Soft Pulses
4. Miscellaneous
5. Conditions for pulse determination:

In principle: Do NOT trust these values blindly - verify them if questionable!

Amplifiers can go bad or your sample environment like salt, temperature, ... might alter the pulse width!

IMPORTANT POWER REFERENCES FOR CRYOPROBES (full document)
(There is no manual for QCI probe available yet)

4.2 Pulse and power recommendations

Note: All values require a minimum repetition period d1 +aq of 1sec ²⁾

Important: observe the separate notes on the foIIowing pages!

Important: Be CAREFUL when pushing these limits!

	5mm 600 MHz TCI CryoProbe ^TM
¹H
Hard pulse ³⁾ (max. length 360^o)	8 usec
Hard pulse for lossy sample	Power level corresponding to 8.0 usec pulse for non-lossy samples
trim pulse p28 ⁴⁾	2 msec @ 10 usec
TOCSY spin lock	200 msec @ 25 usec 400 msec @ 35 usec
ROESY spin lock	Up to CW for power level corresponding to a 100 usec pulse
WALTZ 16 decoupling during 13C-detection	Up to CW for power level corresponding to a 100 usec pulse
DIPSI2-decoupling in triple resonance	400 msec @ 35 usec
¹³C
hard pulse ⁶⁾ (max. length 360^o)	12.0 usec
trim pulse ⁵⁾	2msec @ 25 usec
CC spin lock ⁵⁾	20msec @ 25 usec
GARP decoupling ⁷⁾	140msec @ 55 usec
selective pulses ⁸⁾	Q5: 320 usec Q3: 256 usec CHIRP: 2ms @ 25 usec
¹⁵N
hard pulse ⁶⁾ (max. length 360^o)	35.0 usec
GARP4 decoupling ⁷⁾	140 msec @ 170 usec
CPMG T2 ⁹⁾	250 msec @ 52 usec (see warning ⁹⁾)
²H
hard pulse (max. length 360^o)	68 usec
WALTZ16 decoupling	100 msec @ 250 usec
Z-Gradient
Absolute max. current ¹⁰⁾	10 A
Max. overall length ¹¹⁾	10 ms @ 10A

Valid from the date of shipment: January first 2006

1. General Information:

Shimfile: chloshim.600 is a good starting point for aqueous samples
Read Pulses from Master List:
- Read in pulse sequence and Parameter file
- Use the getprosol command or click on the test tube symbol in ased
- For Bruker Pulse Sequences type: getprosol 1H 'p1' ' pldb1' , where p1 is the determined pulse length [us] at the power level pldb1 [dB].

2. Hard Pulses:

Nucleus	Type	Attenuation	Channel	90deg Pulse	Power [W]	Calc.	Date
1H		-8.50 dB	f1	9.0 us	7.08 W		12/20/19
	waltz16		f1	70.0 us
	TOCSY		f1	26.0 us
	ROESY		f1	90.0 us
					.
13C	hard	-20.65 dB	f2	12.0 us	116.1 W		12/20/19
AcGly	cpd	-6.70 dB	f2	60.0 us	4.68 W	-6.67 dB	12/20/19
	TOCSY	-14.30 dB	f2	25.0 us	26.9 W	-14.27 dB	12/20/19
	2nd cpd	-3.70 dB	f2	84.6 us	2.34 W	-3.69 dB	12/20/19
	low pow cpd	-0.60 dB	f2	120.0 us	1.15 W	-0.65 dB	12/20/19
	Het TOCSY med sens	-7.90 dB	f2	52.0 us	6.17 W	-7.91 dB	12/20/19
	Het TOCSY high sens	-4.40 dB	f2	78.0 us	2.75 W	-4.39 dB	12/20/19
	Het TOCSY super sens	+5.90 dB	f2	250.0 us	0.26 W	+5.73 dB	12/20/19
					.
15N	hard	-21.20 dB	f3	32.0 us	131.8 W		12/20/19
		-4.50 dB	f3	220.0 us	2.82 W	-4.45 dB	12/20/19
		-1.50 dB	f3	310.0 us	1.41 W	-1.48 dB	12/20/19
		-0.50 dB	f3	350.0 us	1.12 W	-0.42 dB	12/20/19
		-13.60 dB	f3	78.0 us	22.9 W	-13.46 dB	12/20/19
					.
31P	hard	-13.00 dB	f1	40.0 us	19.95 W	fully T/M	9/16/17
		+3.75 dB	f1	250.0 us	0.42 W		9/16/17
		-4.60 dB	f4	100.0 us	2.88 W		9/16/17
		+3.50 dB	f4	250.0 us	0.44 W		9/16/17
					.
2H		-17.20 dB	f1	68.0 us	52.5 W		9/15/17
Urea		-5.40 dB	f1	250 us	3.47 W		9/15/17

3. Soft Pulses:

Nucleus	Type	Channel	Pulse	Attenuation (Calculated)	Power [W]	Resonance	Date
13C , Cali/CO;	Q5_sebop.1 350us	f2	90 deg	-17.70 dB (-17.85)	58.9 W	on	12/20/19
Cali/CO	Q5tr_sebop.1 350us	f2	90 deg	-17.80 dB (-17.85)	60.3 W	on	12/20/19
Cα	Q5.1000 1500us	f2	90 deg	-3.60 dB (-3.98)	2.29 W	on	12/20/19
Cα	Q5tr.1000 1500us	f2	90 deg	-3.95 dB (-3.98)	2.48 W	on	12/20/19
Cali/CO	Q3_surbop.1 270us	f2	180 deg	-17.75 dB (-18.01)	59.6 W	on res	12/20/19
Ca sel	Q3_surbop.1 1050us	f2	180 deg	-5.80 dB (-6.21)	3.80 W	on res	12/20/19
med sel	Q3_surbop.1 740us	f2	180 deg	-8.95 dB (-9.25)	7.85 W	on res	12/20/19
Ca or Co dec	Q3_surbop.1 810us	f2	180 deg	-8.15 dB (-8.47)	6.53 W	on res	12/20/19
13C	direct detect
13C	hard, 12us	f1		-20.30 dB	107.2 W		8/29/14
Cali/CO	Q5.1000 320us	f1	90 deg	-17.60 dB (-17.05)	57.5 W	on	8/29/14
Cali/CO	Q5tr.1000 320us	f1	90 deg	-17.90 dB (-17.05)	61.7 W	on	8/29/14
Cα	Q5.1000 1500us	f1	90 deg	-3.70 dB (-3.63)	2.34 W	on	8/29/14
Cα	Q5.1000 1500us	f1	90 deg	-4.1 dB (-3.63)	2.51 W	on	8/29/14
Cali/CO	Q3.1000 256us	f1	180 deg	-16.30 dB (-16.13)	42.7 W	on	8/29/14
Cali/CO	Q3.1000 700us	f1	180 deg	-4.3 dB (-4.30)	2.69 W		8/29/14
Cali/CO	Q3.1000 1000us	f1	180 deg	-7.45 dB (-7.40)	5.56 W		8/29/14

4. Miscellaneous:

Offset Ca - carbonyl (38 - 174ppm):	136 ppm
1H offset @ 4.7 pppm:	2820 Hz
Main Field:	600.21 MHz
BSMS Field:	+1317 units (10-13-2003)
BSMS Field:	-3891 units (03-03-2011)

5. Conditions for pulse determination:

Sample:
1H-pulses: 2mM Sucrose + 0.5mM DSS + 2mM NaN3, 90% H2O 10% D2O
X-pulses: 13C, 15N labled Urea in DMSO
13C-shift: 48.5ppm, J=138.5Hz
15N-shift: 76.0ppm, J=88.5Hz
or:

13C: AcGly in D2O: 13C-shift: 21.0ppm, J=128.0Hz, offset = 136ppm

Temperature: 298K
NO RF-Filters:
NO attenuators at amplifier input: none