602: 5mm BBI-Z, #Z814601-0107

In principle: Do NOT trust these values blindly - verify them if questionable!

Amplifiers can go bad or your sample environment like salt, temperature, ... might alter the pulse width!

1. General Information:

  • Shimfile: chloshim.600 is a good starting point for aqueous samples

1. General Information:

  • Shimfile: chloshim.600 is a good starting point for aqueous samples
  • Read Pulses from Master List:
    • Read in pulse sequence and Parameter file
    • Use the getprosol command or click on the test tube symbol in ased
    • For Bruker Pulse Sequences type: getprosol 1H 'p1' ' pldb1' , where p1 is the determined pulse length [us] at the power level pldb1 [dB].

2. Hard Pulses:

70 us 25us 90 us
Nucleus Type 90 deg Pulse Channel Attenuation Power [W] Calc. Date
1H 8.8 us f1 -10.00 10.0 W Max: 20W 08/29/20
waltz16 f1
TOCSY f1
ROESY f1
.
13C hard 12.50 us f2 -20.95 dB 124.5 W Max: 160W 08/29/20
Urea cpd 60 us f2 -7.30 dB 5.37 W -7.33 dB 08/29/20
TOCSY 25 us f2 -14.95 dB 31.3 W -14.93 dB 08/29/20
2nd cpd 84.6 us f2 -4.30 dB 2.69 W -4.34 dB 08/29/20
low pow cpd 120 us f2 -1.30 dB 1.35 W -1.30 dB 08/29/20
Het TOCSY med sens 52 us f2 -8.55 dB 7.16 W -8.57 dB 08/29/20
Het TOCSY high sens 78 us f2 -5.00 dB 3.16 W -5.05 dB 08/29/20
.
15N hard 26.5 us f3 -21.10 dB 128.8 W Max: 180W 08/29/20
Urea 220 us f3 -2.60 dB 1.82 W -2.72 dB 08/29/20
310 us f3 +0.30 dB 0.93 W +0.26 dB 08/29/20
350 us f3 +1.20 dB 0.76 W +1.32 dB 08/29/20
80 us f3 -11.65 dB 14.6 W -11.50 dB 08/29/20
.
31P hard 20.0 us f1 -17.50 dB 56.2 W Max: 100W 6/5/20
TPP 100 us f1 -3.50 dB 2.24 W 6/5/20
.
2H 80 us f1 -13.30 dB 21.4 W Max: 30W 8/30/20
Urea 250 us f1 -3.60 dB 2.29 W 8/30/20

3. Soft Pulses:

Nucleus Type Channel Pulse Attenuation (Calculated) Power [W] Resonance Date
13C , Cali/CO; Q5_sebop.1 350us f2 90 deg -18.30 dB (-18.50) 67.6 W on 08/29/20
Cali/CO Q5tr_sebop.1 350us f2 90 deg -18.60 dB (-18.50) 72.4 W on 08/29/20
Cα Q5.1000 1500us f2 90 deg -4.35 dB (-4.64) 2.72 W on 08/29/20
Cα Q5tr.1000 1500us f2 90 deg -4.65 dB (-4.64) 2.92 W on 08/29/20
Cali/CO Q3_surbop.1 270us f2 180 deg -18.55 dB (-18.66) 71.6 W on res 08/29/20
Ca sel Q3_surbop.1 1050us f2 180 deg -6.80 dB (-6.87) 4.79 W on res 08/29/20
med sel Q3_surbop.1 740us f2 180 deg -9.70 dB (-9.91) 9.33 W on res 08/29/20
Ca or Co dec Q3_surbop.1 810us f2 180 deg -8.90 dB (-9.12) 7.76 W on res 08/29/20

4. Miscellaneous:

Offset Ca - carbonyl (38 - 174ppm): 136 ppm
1H offset @ 4.7 pppm: 2820 Hz
Main Field: 600.13 MHz
BSMS Field: +1317 units (10-13-2003)
BSMS Field: -3891 units (03-03-2011)
Main Field: 600.03 MHz
BSMS Field: +5200 units (8-29-2020)

5. Conditions for pulse determination:

  • Sample:
    1H-pulses: 2mM Sucrose + 0.5mM DSS + 2mM NaN3, 90% H2O 10% D2O
    X-pulses: 13C, 15N labled Urea in DMSO
    13C-shift: 48.5 ppm, J=138.5Hz
    15N-shift: 76.0 ppm, J=88.5Hz
    or:
    13C: AcGly in D2O: 13C-shift: 21.0ppm, J=128.0Hz, offset = 136ppm

    31P-shift: -22.91 ppm, Triphenyl Phosphate (TPP)

  • Temperature: 298K
  • NO RF-Filters:
  • NO attenuators at amplifier input: none