601: 5 mm TXI 1H-13C/15N/D XYZ-GRD #Z8323/142

In principle: Do NOT trust these values blindly - verify them if questionable!

Amplifiers can go bad or your sample environment like salt, temperature, ... might alter the pulse width!

1. General Information:

  • Shimfile: chloshim.600 is a good starting point for aqueous samples
  • Read Pulses from Master List:
    • Read in pulse sequence and Parameter file
    • Use the getprosol command or click on the test tube symbol in ased
    • For Bruker Pulse Sequences type: getprosol 1H 'p1' ' pldb1' , where p1 is the determined pulse length [us] at the power level PldB1 [dB].

    2. Hard Pulses:

    Nucleus Type Attenuation[dB] Channel 90deg Pulse Power[W] Calc. Date
    1H -13.0 dB f1 8.00 us 19.95 W 3/24/16
    waltz16 f1 70.0 us 100W
    TOCSY f1 25 us 100W
    ROESY f1 125.0 us 100W
    .
    13C hard -23.90 dB f2 12.0 us 245.5W 3/24/16
    Urea TOCSY -17.70 dB f2 25.0 us 58.9 W -17.52 dB 3/24/16
    AcGly TOCSY -17.35 dB f2 26.0 us 54.3 W -17.18 dB 3/24/16
    bilev cpd -16.10 dB f2 30.0 us 40.7 W -15.94 dB 3/24/16
    cpd -10.2 dB f2 60.0 us 10.5 W -9.92 dB 3/24/16
    LowDec 1 -7.20 dB f2 85.0 us 5.25 W -6.90 dB 3/24/16
    LowDec 2 -4.25 dB f2 120.0 us 2.66 W -3.90 dB 3/24/16
    .
    15N hard -24.95 dB f3 36.0 us 312.6 W 3/24/16
    -17.15 dB f3 90.0 us 51.9 W -16.99 dB 3/24/16
    -8.75 dB f3 240.0 us 7.50 W -8.47 dB 3/24/16
    -5.40 dB f3 350.0 us 3.47 W -5.19 dB 3/24/16
    .
    2H -13.00 dB f1 131.0 us 19.95 W 3/23/16
    Urea -7.40 dB f1 250 us 5.48 W 3/23/16

    3. Soft Pulses:

    Nucleus Type Channel Pulse Attenuation [dB] Power [W] Resonance Date
    13C , Cali/CO Q5.1000 320us f2 90 deg -20.55 dB (-20.65 dB)113.5 W on 3/24/16
    Cali/CO Q5tr.1000 320us f2 90 deg -20.70 dB (-20.65)117.5W on 3/24/16
    Cali/CO Q5.1000 1500us f2 90 deg -7.30 dB (-7.23)5.37 W on 3/24/16
    Cali/CO Q5tr.1000 1500us f2 90 deg -7.60 dB (-7.23)5.75 W on 3/24/16
    Cali/CO Q3.1000 256us f2 180 deg -19.75 dB (-19.73)94.4 W on 3/24/16
    CO dec Q3.1000 768us f2 180 deg -10.40 dB (-10.19)11.0 W on 3/24/16
    Cα Q3.1000 1000us f2 180 deg -8.05 dB (-7.90)6.38 W on 3/24/16

    4. Miscellaneous:

    Magic angle on GREAT III: factor Z/XY = N/A
    Offset Ca - carbonyl (56 - 175ppm): -18109 Hz
    1H offset @ 4.7 pppm: 2820 Hz
    Main Field: 600.21 MHz
    BSMS Field: -432 units (10-10-2003)

    5. Conditions for pulse determination:

    • Sample: 13C, 15N labled Urea in DMSO
      13C-shift: 48.5ppm, J=138.5Hz
      15N-shift: 76.0ppm, J=88.5Hz
      Acetyl Glycine 13C/15N, in D2O
      X-pulses: 13C, 15N labled Acetyl Glycine
      13C-shift: 22.1ppm, J=128.6Hz (1H 1.96 ppm )
      15N-shift: 76.0ppm, J=88.5Hz
    • Temperature: 298K
    • RF-Filters: none
    • Attenuators at amplifier input:
      1H = none
      X = none
      Y = none
      Z = none