601: 5mm QXI-XYZ, #8375-0023

In principle: Do NOT trust these values blindly - verify them if questionable!

Amplifiers can go bad or your sample environment like salt, temperature, ... might alter the pulse width!

1. General Information:

  • Shimfile: chloshim.600 is a good starting point for aqueous samples
  • Read Pulses from Master List:
    • Read in pulse sequence and Parameter file
    • Use the getprosol command or click on the test tube symbol in ased
    • For Bruker Pulse Sequences type: getprosol 1H 'p1' ' pl1' , where p1 is the determined pulse length [us] at the power level pl1 [dB].

2. Hard Pulses:

Nucleus Type Attenuation Channel 90deg Pulse Amplifier Calc. Date
1H 0.0 dB f1 8.4 us 100W 05/10/10
waltz16 f1 100.0 us 100W
TOCSY f1 25-35.0 us 100W
ROESY f1 100.0 us 100W
.
13C hard -3.40 dB f2 15.0 us 500W 05/10/10
AcGly TOCSY +0.35 dB f2 25.0 us 500W dB 05/10/10
TOCSY +2.45 dB f2 32.0 us 500W dB 05/10/10
Dec +8.50 dB f2 65.0 us 500W dB 05/10/10
LowDec 1 +10.40 dB f2 80.0 us 500W dB 05/10/10
LowDec 2 + 16.80 dB f2 160.0 us 500W dB 05/10/10
.
15N hard -2.40 dB f3 45.0 us 500W 05/10/10
Dec +3.30 dB f3 90.0 us 500W dB 05/10/10
Dec +10.20 dB f3 200.0 us 500W dB 05/10/10
Dec +11.75 dB f3 250.0 us 500W dB 05/10/10
.
31P hard 0 dB f1 25.8 us 500Wfully T/M 8/11/2008
dB f2 us 500W
.
2H -6 dB 2HTX 190 us 20W Not tunable 06/22/06

3. Soft Pulses:

Nucleus Type Channel Pulse Attenuation Amplifier Resonance Date
13C , Cali/CO; Q5.1000 320us f2 90 deg -2.15 dB500W on 05/10/10
Cali/CO Q5tr.1000 320us f2 90 deg -2.50 dB500W on 05/10/10
Cα Q5.1000 1500us f2 90 deg +10.75 dB500W on 05/10/10
Cα Q5tr.1000 1500us f2 90 deg +10.25 dB500W on 05/10/10
Cali/CO Q3.1000 256us f2 180 deg -1.55 dB500W on res 05/10/10
Cali/CO Q3.1000 256us f2 180 deg -1.65 dB500W off res 05/10/10
Cali/CO Q3.1000 360us f2 180 deg 1.3 dB500W on res 05/10/10
Cali/CO Q3.1000 360us f2 180 deg 1.25 dB500W off res 05/10/10
CO dec Q3.1000 768us f2 180 deg +7.95 dB500W on 05/10/10
Cα Q3.1000 1000us f2 180 deg +10.55 dB500W on 05/10/10
CO Sinc1.1000 160us f2 90 offs=0ppm +12.60 dB500W 8-31-06
CO Sinc1.1000 160us f2 90 offs=136ppm +12.60 dB500W 8-31-06
CO Sinc1.1000 160us f2 180 offs=0ppm +6.5 dB500W 8-31-06
CO Sinc1.1000 160us f2 180 offs=136ppm +6.60 dB500W 8-31-06
Seduce seduce1c6 330us delta=119ppm f2 90 offs=119ppm 4.6 dB500W 08-31-06
seduce1c6 300us delta=132ppm f2 90 offs=132ppm 4.2 dB500W 08-31-06
adiabatic:α+β hsec.3 500us f2 180 deg dB500W
adiabatic: CO sech1024_4.8 600us f2 180 deg dB500W

4. Miscellaneous:

Magic angle on GREAT III: factor Z/XY = 1.22 (3-30-2002)
Offset Ca - carbonyl (56 - 175ppm): -18109Hz
1H offset @ 4.7 pppm: 2820 Hz
Main Field: 600.21 MHz
BSMS Field: +1317 units (10-13-2003)

5. Conditions for pulse determination:

  • Sample:
    1H-pulses: 2mM Sucrose + 0.5mM DSS + 2mM NaN3, 90% H2O 10% D2O
    X-pulses: 13C, 15N labled Urea in DMSO
    13C-shift: 48.5ppm, J=138.5Hz
    15N-shift: 76.0ppm, J=88.5Hz
    or:
    13C: AcGly in D2O: 13C-shift: 21.0ppm, J=128.0Hz

  • Temperature: 298K
  • RF-Filters:
    1H = none
      X = Highpass 600MHz 13C-Pass; 2H-Stop
      Y = Lowpass 600MHz 15N-pass; Bandstop 2H-Stop
      Z = none
  • Attenuators at amplifier input: none
    1H = 0dB      Serial #: W1301845 / 0344
      X = 1dB      Serial #: W1345043 / 0052
      Y = 2dB      Serial #: W1345043 / 0051
      Z = 1dB      Serial #: W1345043 / 0050