502: 5 mm CPQCI 1H/19F-31P/13C/15N/D Z-GRD #Z153532/0002

In principle: Do NOT trust these values blindly - verify them if questionable!

Amplifiers can go bad or your sample environment like salt, temperature, ... might alter the pulse width!

IMPORTANT POWER REFERENCES FOR CRYOPROBES (full document)
(There is no manual for QCI probe available yet)

4.2 Pulse and power recommendations

 

Note: All values require a minimum repetition period d1 +aq of 1sec 2)

Important: observe the separate notes on the foIIowing pages!

Important: Be CAREFUL when pushing these limits!

 

 

5mm 500 MHz TCI CryoProbe TM

1H

 

Hard pulse 3)

(max. length 360o)

8 usec

Hard pulse for lossy sample

Power level corresponding to 8.0 usec pulse for non-lossy samples

trim pulse p28 4)

2 msec @ 10 usec

TOCSY spin lock

200 msec @ 25 usec

400 msec @ 35 usec

ROESY spin lock

Up to CW for power level

corresponding to a 100 usec pulse

WALTZ 16 decoupling

during 13C-detection

Up to CW for power level corresponding to a 100 usec pulse

DIPSI2-decoupling in triple resonance

400 msec @ 35 usec

13C

 

hard pulse 6)

(max. length 360o)

12.0 usec

trim pulse 5)

2msec @ 25 usec

CC spin lock 5)

20msec @ 25 usec

GARP decoupling 7)

140msec @ 65 usec

selective pulses 8)

Q5: 384 usec

Q3: 307 usec

CHIRP: 2ms @ 25 usec

15N

 

hard pulse 6)

(max. length 360o)

35.0 usec

GARP4 decoupling 7)

140 msec @ 200 usec

CPMG T2 9)

250 msec @ 63 usec (see warning 9))

2H

 

hard pulse

(max. length 360o)

68 usec

WALTZ16 decoupling

100 msec @ 250 usec

Z-Gradient

 

Absolute max. current 10)

10 A

Max. overall length 11)

10 ms @ 10A

 

Valid from the date of shipment: January first 2006

1. General Information:

  • Shimfile: chloshim.600 is a good starting point for aqueous samples
  • Read Pulses from Master List:
    • Read in pulse sequence and Parameter file
    • Use the getprosol command or click on the test tube symbol in ased
    • For Bruker Pulse Sequences type: getprosol 1H 'p1' ' pldb1' , where p1 is the determined pulse length [us] at the power level pldb1 [dB].

2. Hard Pulses:

Nucleus Type Attenuation Channel 90deg Pulse Power [W] Calc. Date
1H -10.00 dB f1 9.0 us 10.0 W 3/4/19
waltz16 f1 80.0 us
TOCSY f1 30.0 us
ROESY f1 90.0 us
.
13C hard -19.80 dB f2 12.0 us 95.5 W 3/4/19
Dec -4.60 dB f2 70.0 us 2.88 W (-4.48 dB) 3/4/19
AcGly -13.55 dB f2 25.0 us 22.7 W (-13.4 dB) 3/4/19
TOCSY -1.40 dB f2 100.0 us 1.38 W (-1.38 dB) 3/4/19
LowDec 2 +1.50 dB f2 140.0 us 0.71 W (+1.54 dB) 3/4/19
-7.20 dB f2 52.0 us 5.25 W (-7.06 dB) 3/4/19
+6.55 dB f2 250.0 us 0.22 W (+6.58 dB) 3/4/19
.
15N hard -19.40 dB f3 34.0 us 87.1 W 3/4/19
Dec -3.05 dB f3 220.0 us 2.02 W (-3.18 dB) 3/4/19
Dec -4.75 dB f3 180.0 us 2.99 W (-4.92 dB) 3/4/19
Dec -0.10 dB f3 310.0 us 1.02 W (-0.20 dB) 3/4/19
Dec -12.10 dB f3 80.0 us 16.2 W (-12.0 dB) 3/4/19
.
31P hard -10.0 dB f1 34.0 us 10.0 Wfully T/M 3/2/19
-0.80 dB f2 100.0 us 1.20 W -0.63 dB 3/2/19
+1.90 dB f1 140.0 us 0.65 W +2.29 dB 3/2/19
.
19F (through BB) -9.50 dB f4 10.0 us 8.91 W 3/2/19
.
2H -19.00 dB f4 68.0 us 79.4 W 3/2/19
-7.70 dB f4 250.0 us W 3/2/19

3. Soft Pulses:

Nucleus Type Channel Pulse Attenuation (Calculated) Power [W] Resonance Date
13C Q5_sebop.1 420us f2 90 deg -15.35 dB (-15.42)34.3 W 3/5/19
Q5tr_sebop.1 420us f2 90 deg -15.70 dB (-15.42)37.2 W 3/5/19
Q5.1000 1800us f2 90 deg -1.45 dB (-1.55)1.40 W 3/5/19
Q5tr.1000 1800us f2 90 deg -1.60 dB (-1.55)1.45 W 3/5/19
Q3_surbop.1 324us f2 180 deg -15.50 dB (-15.6)35.5 W 3/5/19
Q3_surbop.1 1260us f2 180 deg -3.60 dB (-3.78)2.29 W
3/5/19
Q3_surbop.1 888us f2 180 deg -6.75 dB (-6.82)4.73 W 3/5/19
Q3_surbop.1 2520us f2 180 deg +2.50 dB (+2.24)0.56 W 3/5/19
Q3_surbop.1 972us f2 180 deg -5.90 dB (-6.03)3.89 W 3/5/19

5. Conditions for pulse determination:

  • Sample:
    1H-pulses: 2mM Sucrose + 0.5mM DSS + 2mM NaN3, 90% H2O 10% D2O
    X-pulses: 15N + 2H labled Urea in DMSO
    15N-shift: 76.0ppm, J=88.5Hz
    13C: NaAc in D2O
    13C-shift: 21.0ppm, J=128.0Hz
  • Temperature: 298K
  • RF-Filters: none
    1H = none
    X = none
    Y = none
    Z = none
  • Attenuators at amplifier input: none

    POWERCHECK DONE

    1H = 0dB
    X = 0dB
    Y = 0dB
    Z = 0dB
  • Cortab combinations done 1/16/04:
    1H --> FCU 1+2, MOD, MULT, BLA
    13C --> FCU 1, 2, 3, 4, on X-Amplifier: MOD, MULT, BLA, FCU4 on Z amplifier BLA
    15N --> FCU 3 on Y-Amplifier: MOD, MULT, BLA, FCU4 on Z amplifier BLA