502: 5mm TXI-Z, #Z8281-0167
In principle: Do NOT trust these values blindly - verify them if questionable!
Amplifiers can go bad or your sample environment like salt, temperature, ... might alter the pulse width!
This probe is comparable to the Z8281/165 probe, but has a slightly worse sucrose lineshape and some minor cross-talk between the proton and deuterium channel during tuning only.
1. General Information:
- Shimfile: sucrose_167 is a good starting point for aqueous samples
2. Hard Pulses:
| Nucleus | Type | Attenuation | Channel | 90deg Pulse | Amplifier | Date |
|---|
| 1H | | 2.0 dB | f1 | 9.0 us | 100W | 04-07-2010 |
| waltz16 | dB | f1 | 80.0 us | 100W | |
| TOCSY | | f1 | 25-35.0 us | 100W | |
| ROESY | dB | f1 | 100.0 us | 100W | |
| | | | | . | |
| 13C | hard | -3.45 dB | f2 | 15.0 us | 300W | 04-07-2010 |
| TOCSY | +1.0 dB | f2 | 25.0 us | 300W | 04-07-2010 |
| Dec | +2.3 dB | f2 | 29.0 us | 300W | 04-07-2010 |
| LowDec 1 | +9.3 dB | f2 | 65.0 us | 300W | 04-07-2010 |
| LowDec 2 | +11.1 dB | f2 | 80.0 us | 300W | 04-07-2010 |
| | | | | . | |
| 15N | hard | -4.50 dB | f3 | 45.0 us | 300W | |
| | +1.10 dB | f3 | 90.0 us | 300W | 04-07-2010 |
| | +8.15 dB | f3 | 200.0 us | 300W | |
| | +10.10 dB | f3 | 250.0 us | 300W | 04-07-2010 |
| | | | | . | |
| 2H | | dB | 2HTX | us | 20W | |
| | dB | 2HTX | us | 20W | |
| | dB | 2HTX | 300 us | 20W | |
3. Soft Pulses:
| Nucleus | Type | Channel | Pulse | Attenuation | Amplifier | Calc. | Date |
|---|
| 13C | G4 400us | f2 | 90 deg | - dB | 300W | | |
| G4 640us | f2 | 90 deg | + dB | 300W | | |
| G3 256us | f2 | 180 deg | - dB | 300W | | |
| G3 768us | f2 | 180 deg | + dB | 300W | | |
| G4tr 409us | f2 | 90 deg | - dB | 300W | | |
| Sinc1 160us | f2 | 90 deg | + dB | 300W | | |
| Sinc1 160us | f2 | 180 deg | + dB | 300W | | |
| 1H | sinc1.1000 1.2ms | f1 | 180 deg | + dB | 100W | | |
4. Miscellaneous:
| Offset Ca - carbonyl (56 - 175ppm): | Hz |
| 1H offset @ 4.7 pppm: | 2350.24 Hz |
| Main Field: | 500.13 MHz |
| BSMS Field: | +2824 units (04/07/2010) |
5. Conditions for pulse determination:
- Sample:
- 1H-pulses: 2mM Sucrose + 2mM NaN3, 90% H2O 10% D2O
- X-pulses: 13C, 15N labled Urea in DMSO
- 13C-shift: 48.5ppm, J=138.5Hz
- only carbonyl is labled, o1p = 160.58ppm, f1 direct, f2 with zgf2
- 15N-shift: 76.0ppm, J=88.5Hz
- Temperature: 298K
- RF-Filters:
- HPPR-2 is used, hence no filters in line
- Attenuators at amplifier input: none
- 1H = 0dB
- X = 0dB
- Y = 0dB
- Cortab combinations done 10/24/2008, TS 1.3:
- Cortab from Z8281/165 prbe used:
- - QTP_8281_165
- - cortab_8281_165
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