501: 5mm TXI-Z, #Z8281-0167

In principle: Do NOT trust these values blindly - verify them if questionable!

Amplifiers can go bad or your sample environment like salt, temperature, ... might alter the pulse width!

1. General Information:

  • Shimfile: 8341_0011ws.shim is a good starting point for aqueous samples

2. Hard Pulses:

Nucleus Type Attenuation Channel 90deg Pulse Amplifier Calc. Date
1H 0.0 dB f1 9.0 us 100W 10-18-04
waltz16 dB f1 80.0 us 100W dB
TOCSY f1 25-35.0 us 100W
ROESY dB f1 100.0 us 100W dB
.
13C hard -3.3 dB f2 15.0 us 300W 10-20-04
TOCSY 3.1 dB f2 32.0 us 300W +3.28 dB 10-20-04
Dec 7.5 dB f2 54.0 us 300W 7.65 dB 10-20-04
LowDec 1 10.9 dB f2 80.0 us 300W 10.9 dB 10-20-04
LowDec 2 16.9 dB f2 160.0 us 300W 16.9 dB 10-20-04
.
15N hard f3 40.0 us 300W
-3.9 dB f3 45.0 us 300W 10-18-04
f3 50.0 us 300W
+8.85 dB f3 200.0 us 300W +9.05 dB 10-18-04
.
2H dB 2HTX us 20W
dB 2HTX us 20W
dB 2HTX 300 us 20W

3. Soft Pulses:

Nucleus Type Channel Pulse Attenuation Amplifier Calc. Date
13C G4 400us f2 90 deg - dB300W
G4 640us f2 90 deg + dB300W
G3 256us f2 180 deg - dB300W
G3 768us f2 180 deg + dB300W
G4tr 409us f2 90 deg - dB300W
Sinc1 160us f2 90 deg + dB300W
Sinc1 160us f2 180 deg + dB300W
1H sinc1.1000 1.2ms f1 180 deg + dB100W

4. Miscellaneous:

Offset Ca - carbonyl (56 - 175ppm): Hz
1H offset @ 4.7 pppm: 2351 Hz
Main Field: 500.13 MHz
BSMS Field: +8369 units (12-24-2003)

5. Conditions for pulse determination:

  • Sample:
    1H-pulses: 2mM Sucrose + 2mM NaN3, 90% H2O 10% D2O
    X-pulses: 13C, 15N labled Urea in DMSO
    13C-shift: 48.5ppm, J=138.5Hz
    only carbonyl is labled, o1p = 160.58ppm, f1 direct, f2 with zgf2
    15N-shift: 76.0ppm, J=88.5Hz
  • Temperature: 298K
  • RF-Filters:
    1H = 1H Bandpass, 2H Stop
    X = 0 - 31P Lowpass Filer, rejects 1H
    Y = 0 - 15N Lowpass Filer, 2H Stop
    2H = 2H Pass, 15N Stop
  • Attenuators at amplifier input: none
    1H = 0dB
    X = 0dB
    Y = 0dB
  • Cortab combinations done 3-7-2002 XWIN-NMR 3.0:
    1H --> FCU 1+2, BLA
    13C --> FCU 1+2, X-Amplifier: BLA
    15N --> FCU 3, Y-Amplifier: BLA
    31P --> FCU 1+2, X-Amplifier: BLA