501: 5mm QXI-XYZ, #Z8356-0007
In principle: Do NOT trust these values blindly - verify them if questionable!
Amplifiers can go bad or your sample environment like salt, temperature, ... might alter the pulse width!
1. General Information:
- Shimfile: 8281_165ws.shim is a good starting point for aqueous samples
2. Hard Pulses:
Updated in edprosl 1/28/05, (hard pulses only!)
| Nucleus | Type | Attenuation | Channel | 90deg Pulse | Amplifier | Calc. | Date |
|---|
| 1H | | -1 dB | f1 | 9.4 us | 100W | | 2/26/05 |
| waltz16 | | f1 | 70.0 us | 100W | | |
| TOCSY | | f1 | 25-35.0 us | 100W | | |
| ROESY | | f1 | 100.0 us | 100W | | |
| | | | | . | | |
| 13C | hard | -3.5 dB | f2 | 15.0 us | 300W | | 2/26/05 |
| TOCSY | +3.1 dB | f2 | 32.0 us | 300W | 3.1 dB | 2/26/05 |
| Dec | +7.5 dB | f2 | 54.0 us | 300W | 7.65 dB | 2/26/05 |
| LowDec 1 | +10.9 dB | f2 | 80.0 us | 300W | 11.05 dB | 2/26/05 |
| LowDec 2 | +16.9 dB | f2 | 160.0 us | 300W | 17.05 dB | 2/26/05 |
| | | | | . | | |
| 15N | hard | -5.35 dB | f3 | 45.0 us | 300W | | 2/26/05 |
| | dB | f3 | 50.0 us | 300W | dB | |
| | +6.7 dB | f3 | 200.0 us | 300W | 6.7dB | 2/26/05 |
| | -3.30 dB | f2 | 45.0 us | 300W | | 8/09/05 |
| | +9.4 dB | f2 | 200.0 us | 300W | 9.65 dB | 8/09/05 |
| | | | | . | | |
| 31P | hard | 0 dB | f1 | 15.0 us | 300W | | 2/26/05 |
| | 17.65 dB | f1 | 100.0 us | 300W | 16.50 dB | 2/26/05 |
| | 0.0 dB | f2 | 15.0 us | 300W | | 2/26/05 |
| | 16.4 dB | f1 | 100.0 us | 300W | 16.50 dB | 2/26/05 |
| | | | | . | | |
| 2H | | -6 dB | 2HTX | us | 20W | | |
| | +8 dB | 2HTX | us | 20W | | |
3. Soft Pulses:
| Nucleus | Type | Channel | Pulse | Attenuation | Amplifier | Calc. | Date |
|---|
| 13C | G4 400us | f2 | 90 deg | -1.0 dB | 300W | | 8-04-03 |
| G4 640us | f2 | 90 deg | +3.0 dB | 300W | | 8-04-03 |
| G3 256us | f2 | 180 deg | -2.5 dB | 300W | | 8-04-03 |
| G3 768us | f2 | 180 deg | +7.0 dB | 300W | | 8-04-03 |
| G4tr 409us | f2 | 90 deg | -0.5 dB | 300W | | 8-04-03 |
| Sinc1 160us | f2 | 90 deg | +12.0 dB | 300W | | 8-04-03 |
| Sinc1 160us | f2 | 180 deg | +6.0 dB | 300W | | 8-04-03 |
| 1H | sinc1.1000 1.2ms | f1 | 180 deg | + dB | 100W | | |
4. Miscellaneous:
| Magic angle on GREAT III: | factor Z/XY = 1.39 (4-21-03) |
| Offset Ca - carbonyl (56 - 175ppm): | -14965 Hz |
| 1H offset @ 4.7 pppm: | 2350.6 Hz |
| Main Field: | 500.13 MHz |
| BSMS Field: | +2966 units (10-13-2003) |
5. Conditions for pulse determination:
- Sample:
- 1H-pulses: 2mM Sucrose + 0.5mM DSS + 2mM NaN3, 90% H2O 10% D2O
- X-pulses: 15N + 2H labled Urea in DMSO
- 15N-shift: 76.0ppm, J=88.5Hz
- 13C: NaAc in D2O
- 13C-shift: 21.0ppm, J=128.0Hz
- Temperature: 298K
- RF-Filters: none
- 1H = none
- X = none
- Y = none
- Z = none
- Attenuators at amplifier input: none
- 1H = 3dB
- X = 0dB
- Y = 0dB
- Z = 6dB
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